Figure 4
From: Metabolic stress controls mutant p53 R248Q stability in acute myeloid leukemia cells
The effect of DCA on macroautophagy depends on the p53 status. (a) Autophagy flux after DCA treatment in cell lines with different status of p53. AML cell lines were cultured in the presence of different concentrations of DCA for 24 h followed by 4 h of CQ (50 μM). Autophagy flux was monitored by detection of LC3-II under CQ treatment. (b) Effect of 17-AAG alone or in combination with DCA and different status of p53 on autophagy flux. AML cells were treated with 10 mM DCA, and after one hour, 17-AAG was added (1 μM) for 24 h. Autophagy flux was monitored by the addition of 50 μM CQ for 4 h before collecting the cells for western blot analysis. Graphs represent the quantification of the western blots after giving a standard value of 1 to the control.
