Figure 8
From: Metabolic stress controls mutant p53 R248Q stability in acute myeloid leukemia cells
The combination DCA + 17-AAG causes inhibition of macroautophagy in the presence of R248Q. (a) NB4 cells were transfected with control siRNA and p53 siRNA. Knockdown efficiency was validated by qPCR and western blot. (b) DCA + 17-AAG co-treatment causes inhibition of autophagy flux in presence of R248Q. After 72 h transfection with the indicated siRNAs, NB4 cells were treated with 10 mM DCA and/or 1 µM 17-AAG. Autophagy flux was monitored by detection of LC3-II under CQ treatment. (c) R248Q inhibits autophagy flux stimulated by metabolism stress. HL60 cells were transfected with plasmids encoding wt p53 or R248Q and after 24 h, DCA and/or 17-AAG were added for 24 h. CQ was used to monitor autophagy flux. Graphs represent the quantification of the western blots.
