Figure 3
From: Formation of tubules and helical ribbons by ceramide phosphoethanolamine-containing membranes
Darkfield (A) and fluorescence (B) micrographs of a dispersion of milk CPE:diC18:1 PC:DiI C18 (50:50:0.1) in water. Milk CPE (75 nanomoles in chloroform/methanol), diC18:1 PC (75 nanomoles in chloroform) and DiI C18 (0.15 nanomoles in chloroform) were aliquoted in a glass tube, solvents were removed under N2 flow and films were dried in vacuo as described in Materials and Methods. The lipid suspension was prepared at 60 °C as described in Materials and Methods. Darkfield and fluorescence images do not exactly overlap due to the sample movement between image acquisition. Red arrows indicate structures that are perceived as helical by darkfield and tubular by fluorescence microscopy. Bars, 10 μm.
