Figure 2

Stiffening effect on DNA of nucleosome compared to histone proteins types for reconstituting chromatin. Real time monitoring of the stiffness on SNTs by injecting increasing concentration of (A) histone protein H2A, (B) histone protein H3, (C) histone protein duplex H3/H4, and (D) nucleosome. (E) Histogram of stiffness average after 4 minutes of incubation of either histone protein H2A, or histone protein H3, or histone protein duplex H3/H4, or nucleosome at 100 nM. pH value of each sample is represented by the universal color code of pH paper and indicated in the inset (green for neutral pH, red for acidic pH). pH data from all samples are given in Table S1.