Figure 3
AKT inhibition by MK-2206 synergistically enhances eribulin-induced cytotoxicity in STS cells. (A,B) HT1080, SK-LMS-1 and SW872 cells were incubated with increasing doses of eribulin and MK-2206 for 48 h. Cell growth reduction was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assays. (C,D) HT1080, SK-LMS-1 and eribulin resistant cells (r1 and r2) were treated with or without the indicated concentrations of eribulin, MK-2206, or a combination of these for 48 h. The combination of eribulin plus MK-2206 induced synergistic cytotoxicity in even resistant cell lines, although this was less effective than in parental cell lines. The data represents the mean of four independent cultures. Error bars represents the standard deviation (SD). CI: Combination index. CI < 1: synergistic.
