Figure 4

Combined treatment of eribulin plus MK-2206 triggers G1 or G2/M arrest in STS cells. (A) HT1080 or SK-LMS-1 cells were untreated (vehicle) or treated with eribulin and/or MK-2206 for 24 h, and then stained with propidium iodide. Subsequently, cell cycles were analyzed by flow cytometry. Error bars represent the standard deviation (SD). *P < 0.05, **P < 0.01. Combo: combined eribulin and MK-2206. (B) Immunoblot analysis of cell lysates after treatment of cells with combined eribulin and MK-2206 for 24 h. Cell cycle–regulated proteins were analyzed using the indicated antibodies. The β-actin protein served as a loading control for each experiment. Combo: combined eribulin and MK-2206. These experiments were repeated three times.