Figure 5
Combination treatment of eribulin plus MK-2206 induces caspase-dependent apoptosis of STS cells. (A) HT1080 and SK-LMS-1 cells were treated with IC25 doses of eribulin, MK-2206, or a combination of both (combo), with or without a pan-caspase inhibitor, Q-VD-OPH (20 µM), for 48 h. Q-VD-OPH was added 1 h before eribulin and MK-2206 treatments. Apoptotic cells were analyzed by flow cytometry using annexin V/7-AAD staining. Error bars represent the standard deviation (SD). **P < 0.01. (B) HT1080 and SK-LMS-1 cells were treated with IC25 doses of eribulin, MK-2206, or a combination of both (combo) for 48 h. Activities of caspases 8 and 9 were determined by flow cytometry. These experiments were repeated three times. Error bars represent the SD. *P < 0.05, **P < 0.01.
