Figure 1 | Scientific Reports

Figure 1

From: Aspergillus fumigatus phosphoethanolamine transferase gene gpi7 is required for proper transportation of the cell wall GPI-anchored proteins and polarized growth

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Confirmation of the mutant and revertant by Southern blotting. The null mutant Δgpi7 was constructed by replacing of the gpi7 gene with pyrG and the revertant strain Regpi7 was constructed by introducing of the gpi7 gene into the mutant as described under Methods. Both wild-type (WT) and revertant strains were confirmed by Southern blotting. Probe 1 and probe 2, amplified from the up-stream flanking sequence of the gpi7 and the pyrG gene respectively, were used as probes. Genomic DNA digested with Sal I was probed with Probe 1 (B) or Probe 2 (C) as described under Methods. In the ER, the second mannose of GPI-anchor is modified by Gpi7 with EtN-P and then protein will be attached to GPI anchor. Once GPI anchored protein is formed, the EtN-P on the second mannose will be removed by Ted1p/PGAP5. Then p24 protein, a cargo receptor in COPII vesicle, can recognize the mature GPI-anchored protein by direct binding to the second mannose, which leads to the formation of a specialized COPII vesicle43 (D).

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