Figure 7 | Scientific Reports

Figure 7

From: Aspergillus fumigatus phosphoethanolamine transferase gene gpi7 is required for proper transportation of the cell wall GPI-anchored proteins and polarized growth

Figure 7The alternative text for this image may have been generated using AI.

Localization of GPI anchored proteins. Cell wall GPI proteins (GPI-CWP) were released from the cell wall by hydrofluoride-pyridine (HF); Cell membrane proteins (GPI-PMP) were released from membrane fraction by HF, membrane fraction was prepared by ultracentrifuge. Extracellular proteins were extracted from culture supernatant by precipitation and intracellular proteins were obtained from the cell lysate35. Western blotting was carried out with anti-Gel1, anti-Ecm33, and anti-Mp1 antibody, respectively. Lower levels or degradation of Gel1 (452 amino acids with 2 N-glycosylation sites and some O-glycosylation sites, 56 kDa), Ecm33 (398 amino acids with 9 N-glycosylation sites and some O-glycosylation sites, 61 kDa) and Mp1 (284 amino acids with some potential O-glycosylation sites, 42 kDa) can be observed in certain cellular subfractions.

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