Figure 8

Distribution of the GFP-Mp1 chimeric protein in the Δgpi7 mutant. In (A), plasmid GPDA-Pro::chiB-N-signal-GFP-Mp1-C; in (B), expression of chimeric GFP-Mp1 in the WT and Δgpi7 mutant; and in (C), GFP-Mp1 was detected with Anti-GFP polyclonal antibody (Clontech) by using immuno gold labeling method as described under Material and Methods; in (D), GFP-Mp1 in the Δgpi7 mutant was detected with anti-GFP polyclonal antibody. Gold particles are marked with boxes.