Figure 4
The effects of ITZ on the size distribution of intestinal polyps and on the expression of TCF/LEF, NF-κB and NRF2 target genes in Min mice. (A) Min mice were fed a basal diet (open box), 100 ppm (gray-filled box) and 500 ppm (black-filled box) of an ITZ-containing diet for 8 weeks. The number of polyps per mouse in each class is given as the mean ± SD. *p < 0.05 vs. 0 ppm. (B) Quantitative real-time PCR analysis was performed to determine LGR5, COX-2 and HO-1 mRNA expression levels in the non-polyps or polyps of Min mice with or without 100 ppm ITZ for 8 weeks. The data are normalized to GAPDH expression. Each expression level in the non-polyps of the untreated group was set as 1. The data are the mean ± SD, n = 4. *p < 0.05 vs. 0 ppm.
