Figure 4
From: RNA recognition motifs of disease-linked RNA-binding proteins contribute to amyloid formation
The β2 peptides of TDP-43 RRM1 and FUS RRM are prone to fibril formation. (A) Amyloid-promoting segments (predicted by ZipperDB) in TDP-43 RRM1 and FUS RRM are presented as red and orange bars. Amino acid sequences are listed at the top, with secondary structures (labeled as α and β) derived from the crystal structure of TDP-43 RRM1 (PDB entry: 4Y0F) and the NMR structure of FUS RRM (PDB entry: 2LCW) shown above. Three β2 peptides that formed fibrils are labeled in red, whereas the peptides that did not form fibrils are labeled in black, shown at the bottom of each histogram. (B) The peptide sequences from the β2 region of TDP-43 RRM1 (β2a and β2b) and FUS RRM (β2) form fibrils in vitro, as revealed by negative-stain transmission electron microscopy.
