Figure 5
From: RNA recognition motifs of disease-linked RNA-binding proteins contribute to amyloid formation
Fibril aggregates of TDP-43 RRM1, FUS RRM and their β2 peptides exhibit amyloid properties. (A) Fluorescence spectra (460 to 600 nm, red lines) of the freshly-formed fibrillar solutions of FUS RRM and TDP-43 RRM1 revealed fluorescence signals in the presence of Thioflavin T (excited at 442 nm), but the fresh protein solutions did not generate any signal (black lines). (B) X-ray diffraction images of the fibrils formed by FUS RRM, TDP-43 RRM1 and their β2 peptides (TDP-43 RRM1 β2a, TDP-43 RRM1 β2b and FUS RRM β2) reveal the characteristic cross-β diffraction patterns of amyloid fibrils at 4.7 Å and 10 Å (marked by red rings).
