Figure 3
From: Comparative RNA sequencing reveals that HPV16 E6 abrogates the effect of E6*I on ROS metabolism
HPV16 E6 and E6*I expression differentially alters phenotypic characteristics relevant to cancer cells. (A) Cell proliferation assay with stably transfected cells U-CTL (black), U-E6All (red) and U-E6*I (blue) was performed to monitor daily live cells for 4 days. Cell counts are represented as mean +/− S.D. of 3 independent experiments. (B) Representative images of the colony formation assay realized with U-CTL#par, U-CTL#3, U-E6All#7 and U-E6*I#1. (C) Quantification of the colony formation assay realized with U-CTL (#par, #1, #3), U-E6All (#1, #6 and #7) and U-E6*I (#1, #6 and #14) cell lines. Foci counts are represented as mean +/− S.D. of 3 independent experiments. (D) Detection of H2O2 released from U-CTL (#par, #1, #3), U-E6All (#1, #6 and #7) and U-E6*I (#1, #6 and #14) cell lines in 1 h using the Amplex Red assay. H2O2 relative levels are represented as mean +/− S.D. of 3 independent experiments. (E/F) Clonogenic radio sensitivity assay performed with U-CTL, U-E6All and U-E6*I cell lines. Cell colonies (>50 cells) were counted 10 days following irradiation (0 to 8 Gy). Mann-Whitney test were used to assess statistical significance (*p < 0.05; **p < 0.01; ***p < 0.001).
