Figure 1
CD8+ and CD4+ T cells of donor and recipient origin are present in the renal allograft. Lymphocytes were isolated from the rejected renal allografts and subsequently stained and analysed by flow cytometry. (A) Gating strategy used to detect CD3+ T cells within the total viable lymphocytes, of which the CD8+ and CD4+ T cells were selected. (B) Typical examples of renal lymphocyte samples of patient number three and seven stained with mAb against human leukocyte antigen (HLA) class I antigens within the CD4+ T cells. Proportions of cells originating from the donor are depicted. (C) Table with numbers referring to the renal allografts described in Table 1, the time to explantation in days, and the proportions of donor cells detected within the CD8+ and CD4+ T cell compartment. Frequencies of the cells are presented as individual proportions with medians. HLA = human leukocyte antigen; N.S. = not significant; N/D = not determined.
