Figure 5

SLURP1 suppresses nuclear localization of β-catenin in TNF-α-activated confluent HUVEC monolayer. (A) Immunofluorescent stain with anti-pan β-catenin (upper panels; red), or anti-nuclear β-catenin antibody (lower panels; green) is shown. (B) Fraction of HUVEC with nuclear localization of β-catenin under different conditions tested. Cells with nuclear β-catenin counted by using a program in Metamorph. The data presented is representative of three independent experiments. CP, control protein mock purified from the parental strain lacking SLURP1 expression vector.