Figure 2

RT-QuIC sensitive detection of PrP^L-BSE, PrP^C-BSE and PrP^scrapie from goat brain homogenates. (A) rHaSPrP^Sen 23–231 substrate was used to detect both PrP^L-BSE (azure), PrP^C-BSE (green) and PrP^scrapie (magenta) from brain homogenates. 10⁻⁴ brain tissue dilutions were used to seed quadruplicate RT-QuIC reactions. Normal control brain homogenates (black) showed no response. The number of samples is in parentheses. Representative sensitivity of detection for PrP^L-BSE (shades of azure; B) PrP^C-BSE (shades of green; C) and PrP^scrapie (shades of magenta; D) in brain homogenates (BH) using rHaSPrP^Sen 23–231 as a substrate. Dilutions are indicated next to the curve. (E) rHaPrP^Sen 90–231 substrate was used to detect both PrP^L-BSE (azure) and PrP^C-BSE (green) from brain homogenates. 10⁻⁴ brain tissue dilutions were used to seed quadruplicate RT-QuIC reactions. Normal control brain homogenates (black) showed no response. The number of samples is in parentheses. (F) Representative sensitivity of detection for PrP^C-BSE (shades of green) and PrP^L-BSE (shades of azure) in brain homogenates (BH) using rHaPrP^Sen 90–231 as a substrate. Dilutions are indicated next to the curve. Similar results were obtained from two additional C-BSE-infected and two additional L-BSE-infected brain specimens (presented in Supplementary Fig. S3). Each ThT reading is indicated as the percentage of the maximum value achievable by the plate readers as a function of reaction time.