Figure 3 | Scientific Reports

Figure 3

From: Early and Pre-Clinical Detection of Prion Seeding Activity in Cerebrospinal Fluid of Goats using Real-Time Quaking-Induced Conversion Assay

Figure 3

Comparison of first (PQ-CSF) and second generation (IQ-CSF) of RT-QuIC assays using rHaPrPSen 90–231. (A,B) Two L-BSE-infected (G3 and G4, blue) and uninfected (black) goat CSF were tested at 55 °C by using truncated rHaPrPSen 90–231 with (right) or without (left) the addition of 0.002% SDS. Two human CSF (red) from patients affected with sCJD were used as positive control. Distinct symbols represent separate samples. (C,D) Next, rHaSPrPSen 90–231 substrate was used to detect both PrPC-BSE (green) and PrPscrapie (magenta) from CSF of 3 symptomatic goats (G2, Sc1 and Sc2) using PQ-CSF (C) or IQ-CSF (D) of RT-QuIC assays.

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