Figure 4
Detection of pathological prion protein in CSF of L-BSE infected goats at the asymptomatic stage using IQ-CSF assay. (A) PrPSc detection in CSF of three L-BSE infected, but asymptomatic goats (shades of blue). CSF collected at 926 days post inoculum (dpi), and at clinical onset (shades of orange) from the same L-BSE-infected goat were tested at 55 °C by using truncated rHaPrPSen 90–231 with the addition of 0.002% SDS. Distinct symbols represent separate L-BSE samples. (B) analytical sensitivity of one goat L-BSE CSF collected at the clinical onset using IQ-CSF conditions. Reaction mixtures seeded with serial decreasing volume of L-BSE-infected CSF samples (20- to 0.46 µl equivalents of pure CSF) were tested. Distinct symbols represent separate volume of pure CSF seeded. (C) analytical sensitivity of goat L-BSE-infected CSF collected 407 days before clinical symptoms appeared using IQ-CSF conditions. Reaction mixtures seeded with serial decreasing volume of L-BSE-infected CSF samples (20- to 0.46 µl equivalents of pure CSF) were tested. Distinct symbols represent separate volume of pure CSF seeded.
