Table 1 List of AO names and sequences used in this study.

From: Systematic evaluation of 2′-Fluoro modified chimeric antisense oligonucleotide-mediated exon skipping in vitro

AO name

Sequence, 5′ → 3′ direction

Fully 2′-OMe-PS

GCCAAACCUCGGCUUACC

Fully 2′-F-PS

GFCFCFAFAFAFCFCFUFCFGFGFCFUFUFAFCFCF

2′-OMe/2′-F-PS gapmer

GCCFAFAFAFCFCFUFCFGFGFCFUFUFAFCC

2′-OMe/2′-F-PS mixmer 1

GCFCFAFAFAFCCFUFCFGFGCFUFUFAFCFC

2′-OMe/2′-F-PS mixmer 2

GFCFCFAFAFAFCFCFUCFGFGCFUFUAFCFC

LNA/2′-F-PS gapmer

GLCLCFAFAFAFCFCFUFCFGFGFCFUFUFAFCLCL

LNA/2′-F-PS mixmer 1

GLCFCFAFAFAFCLCFUFCFGFGLCFUFUFAFCFCL

LNA/2′-F-PS mixmer 2

GFCFCFAFAFAFCFCFTLCFGFGLCFUFTLAFCFCL

  1. 2′-OMe nucleotides are represented in underlined and bold letters; 2′-F nucleotides are represented in black letters with superscript F; LNA nucleotides are represented in underlined and bold-italic letters with superscript L. All AOs possess PS backbone.