Figure 1 | Scientific Reports

Figure 1

From: Development of a Phage Display Panning Strategy Utilizing Crude Antigens: Isolation of MERS-CoV Nucleoprotein human antibodies

Figure 1

Schematic diagram of Yin-Yang biopanning method. The procedure involves the following steps, 1. Immunotube blocking with E. coli lysate and skimmed milk overnight, wash before use; 2. Adding the desired amount of antibody phage library into the immunotube; 3. Phage library preincubation takes place for 1 h in the immunotube; 4. Bring over the preincubated phage library into antigen-coated well for binding; 5. Stringent washing and elution of phage with trypsin enzymatic action.

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