Figure 3 | Scientific Reports

Figure 3

From: Development of a Phage Display Panning Strategy Utilizing Crude Antigens: Isolation of MERS-CoV Nucleoprotein human antibodies

Figure 3

Phage titres of biopanning simulations of αUbi and M13KO7 incubated with various blocking agents. αUbi and M13KO7 were separately incubated in four blocking agents (PBS, 2% PTM, E. coli lysate, 2% PTM and E. coli lysate) during binding towards purified rUbi. The phages were collected at the end of binding by trypsin elution. αUbi (left) and M13KO7 (right) were collected before and after elution as ‘Blocked phages’ and ‘Eluted phages’. The data of mean ± s.d. (n = 3) of triplicates is shown.

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