Figure 5 | Scientific Reports

Figure 5

From: Development of a Phage Display Panning Strategy Utilizing Crude Antigens: Isolation of MERS-CoV Nucleoprotein human antibodies

Figure 5

Phage titres of biopanning simulations of αUbi and M13KO7 with lysate preblocking and phage preincubation. αUbi and M13KO7 were separately incubated in the lysate/PTM-preblocked microwells in four different blocking agents (PBS, 2% PTM, E. coli lysate, 2% PTM and E. coli lysate). At the end of preincubation, the phages were transferred to antigen-coated wells for binding at 37 °C, 600 rpm for 2 hours. αUbi (left) and M13KO7 (right) were collected before and after elution as ‘Blocked phages’ and ‘Eluted phages’. The data shows the mean ± s.d. (n = 3) of triplicates.

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