Figure 5
From: Ectopic Dpp signaling promotes stem cell competition through EGFR signaling in the Drosophila testis
Ectopic Vn/EGFR/MAPK signaling is responsible for CySC-GSC competition and GSC loss in c587ts > tkvCA testes. (a) c587ts control testis. (b) c587ts > vnEP testis. Note that most GSCs (red, white arrowheads) are competed out of the niche by CySCs (yellow arrowheads). (c) Quantification of the number of GSCs per testis in the testis with indicated genotype. n = 10–15 testes. (d) Quantification of the number of CySCs with their cell body attaching to the hub per testis with indicated genotypes. n = 10–15 testes. (e) c587ts > tkvCA testes. (f) c587ts > vnRNAi testes (HMS00004). No obvious defects are observed when this shRNA is induced. (g) Stem cell competition observed in c587ts > tkvCA testis is almost completely suppressed by simultaneous expression of this shRNA against vn. (h) c587ts > egfrRNAi testes. The induction of this weak egfrRNAi line (JF01368) in CySCs does not cause any obvious defects. (i) Stem cell competition observed in c587ts > tkvCA testis is greatly suppressed by co-expression of this egfrRNAi. mean ± SEM is shown. **p < 0.01. GFP in green, Vasa in red, Fas3 in yellow, blue indicates DAPI staining for DNA. Scale bars: 10 μm.
