Figure 1

Survival rate of wild-type and IBP-expressing worms after cold shock and freezing. (A) Cold- and freezing-tolerance assays to observe the survival rate of C. elegans. (B,C) Survival rate of each IBP-expressing worm after −5 °C (freezing) exposure and −2 °C and 0 °C (cold shock) exposure. NfeIBPs were isolated from Zoarces elongatus Kner (Notched-fin eelpout). AnpIBP was isolated from Antarctomyces psychrotrophicus. In each assay, n ≧ 20 (group ≧ 5). Error bars indicate the standard error of the mean. A Bonferroni t-test was performed to compare the IBP-expressing worms and wild-type animals. ***p < 0.001, n.s. represents no significance.