Figure 5

TisIBP8 protects body wall muscle cells in C. elegans. (A) Ice crystals in extracts from WT and TisIBP8-expressing worms. The ice crystal shape in the WT extracts is hexagonal, whereas that in the TisIBP8-expressing worm extracts is star-like. (B) Localization of the wrmScarlet signal in body wall muscles. The panel on the right shows an enlarged view of the dotted square in the merged panel. The arrows indicate wrmScarlet puncta from the cell nucleus in body wall muscles. (C,D) Number of cells expressing wrmScarlet in body wall muscle cells in wild-type, AnpIBP and TisIBP8 worms. n ≧ 20. Boxes show the median and first and third quartiles. The Wilcoxon rank sum test was performed. **p < 0.01, ***p < 0.001.