Figure 6
From: c-Myb regulates tumorigenic potential of embryonal rhabdomyosarcoma cells
c-Myb suppression promotes myogenic differentiation of eRMS cells. (a) Western blotting analysis. RD cells transduced with a Dox-inducible c-Myb shRNA (RDshMYB) vector or empty vector (RDEmpty), parental RD cells, or C2C12 myoblasts were cultured with or without Dox, induced to differentiate by incubation in DM for 72 hours, and immunostained for myosin heavy chain (MHC). GAPDH served as a loading control. The original full-length blots are presented in Supplementary Fig. 1. (b) Quantification of MHC specific band in differentiating RD cells.
