Figure 7
Comparison of ARNTL, CLOCK, and AhR genomic enrichment within target genes assessed using chromatin immunoprecipitation (ChIP). Liver samples were collected at ZT4–6 from male C57BL/6 mice 2 h following a single bolus dose of sesame oil vehicle or 30 µg/kg TCDD. Genomic enrichment was quantified by qRT-PCR using primers targeting regions within (A,B) Per1, (C,D) Per2, (E) Dbp, and (F) Nr1d1 as described in Supplementary Table S2. (G) Cyp1a1 was used as a positive control for AhR binding, while (H) a negative control region on chromosome 6 was used to confirm the specificity of each immunoprecipitation. Data points represent the average % input of 4–5 individual mice ± standard error of the mean. Statistical significance (*p ≤ 0.05) between vehicle- and TCDD-treated mice was determined using a t-test.
