Figure 8
Functional analysis of hepatic genes and metabolites which lost their rhythmicity following TCDD treatment. Transcriptomic and metabolomic changes were assessed in male C57BL/6 mice orally gavaged with sesame oil vehicle or 30 µg/kg TCDD every 4 days for 28 days. (A) The Database for Annotation, Visualization, and Integrated Discovery (DAVID) v6.8 was used to identify enriched functional clusters within 2,804 hepatic genes with abolished rhythmicity and TCDD-elicited differential expression at three or more time points. (B) Flow chart summarizing the analysis of an untargeted metabolomics assessment of polar hepatic extracts run in both positive and negative mode. (C) The mummichog algorithm in MetaboAnalyst v4.0 (MS Peaks to Pathways) was used to identify enriched KEGG pathways in the 900 and 1,637 oscillating peaks identified in positive (orange) and negative (blue) mode, respectively, which lost their rhythmicity following TCDD treatment. Scores ≥1.3 (red dotted line) were considered significantly enriched.
