Figure 5
From: Cold-inducible RNA-binding Protein Induces Neutrophil Extracellular Traps in the Lungs during Sepsis
Assessment of PAD4 expression in the lungs during sepsis. Total protein was extracted from lung tissues and PAD4 expression at protein levels was measured by Western blotting. Each blot was quantified by densitometric analysis. PAD4 expression in each sample was normalized to β-actin expression and the mean values of WT sham groups were standardized as one for comparison. Representative blots for PAD4 and β-actin from three independent experiments are shown. The full-length gel from where the cropped bands are taken is displayed in Supplemental Fig. 4, in which the cropped bands are shown in yellow box. Data are expressed as means ± SE (n = WT sham 10, WT CLP 10, CIRP−/− sham 10, and CIRP−/− CLP 10 mice) and compared using one-way ANOVA and SNK method (*p < 0.05 vs WT sham, #p < 0.05 vs WT CLP).
