Figure 2
From: CD271 is a molecular switch with divergent roles in melanoma and melanocyte development
Transcriptional validation of differentially expressed genes by qRT-PCR based on CD271 status and normalization against 18 s RNA using the ΔCT method. The fold change for a transcript of interest was determined by taking the ratio of normalized gene expression between CD271+ and CD271− cell populations for each patient sample. In brief, differential gene expression was determined by calculating the fold change of any gene of interest (GOI) = 2−ΔΔCT, where ΔCT(GOI) = (CT(GOI) − CT(18 s) and ΔΔCT(GOI) = ΔCT(GOI-condition A) − ΔCT(GOI condition B) with CD271+ and CD271− for condition A and B, respectively.
