Figure 6

Compound 13 causes the formation of multiple spindle poles and uneven segregation. (a) HeLa S3 cells were synchronized at G2/M by nocodazole, followed by incubation for 6 h in drug-free medium in the absence (Sham control) or presence of 5 μM of compound 13. Note the presence of uneven cell sizes (arrows) which were likely generated as a result of nondisjuctional chromosome segregation during mitosis. (b) Multiple spindle phenotypes were seen in 44.4% of compound 13-treated cells. In contrast, the untreated control sample contains very few cells with abnormal spindles.