Figure 2
From: Hypergravity and microgravity exhibited reversal effects on the bone and muscle mass in mice
Analysis of triceps surae muscle volume using μCT in 2G mice. (A) The cross-sectional images of lower leg at center rejoin with muscle, were reconstructed by μCT. (B) The triceps surae muscle volume (MV) and MV per body weight (BW) were analyzed by μCT. (C,D) The mRNA expression of myogenic genes (Myod, Myog, Myf5, Myh1 and Myh2), muscle-specific ubiquitin ligases (Atrogin1 and Murf1) and autophagy-related genes (Lc3b, Atg5, Atg7 and Atg16L) in quadriceps were measured using qPCR. In qPCR analysis, all genes were normalized by three reference genes (Actb, 18 s and Hprt). Data are expressed as the mean ± SEM of 7–8 mice. A significant difference between the two groups was indicated; *P < 0.05, **P < 0.01 and ***P < 0.001 vs 1G.
