Figure 3
From: Hypergravity and microgravity exhibited reversal effects on the bone and muscle mass in mice
The μCT analysis for the bone mass of humurus, femur and tibia in 2G mice. The three-dimensional images (horizontal section, isolated trabecular bone of horizontal section, and vertical section) were shown in upper panels, and trabecular bone mass parameters were analyzed by μCT and shown in lower panels in humurus at the proximal region (A), femur at the distal region (B) and tibia at the proximal region (C). The μCT images showed representative bones of 7–8 mice. The mRNA expression of bone formation-related genes (D) and osteoclastogenesis-related genes (E) were measured using qPCR in tibial trabecular bone with bone marrows. In qPCR analysis, all genes were normalized by three reference genes (Actb, 18 s and Hprt). Data are expressed as the mean ± SEM of 7–8 mice. A significant difference between the two groups was indicated; *P < 0.05, **P < 0.01 and ***P < 0.001 vs 1G.
