Table 1 Primer sequences for qPCR.
From: Hypergravity and microgravity exhibited reversal effects on the bone and muscle mass in mice
Genes | Forward | Reverse |
|---|---|---|
mouse Myod | ccttgctcagctccctca | tgggagttgcattcactgg |
mouse Myog | cccatggtgcccagtgaa | gcagattgtgggcgtctgta |
mouse Myf5 | tgaatgtaacagccctgtctggtc | cgtgatagataagtctggagctgg |
mouse Myh1 | gagggacagttcatcgatagcaa | gggccaacttgtcatctctcat |
mouse Myh2 | aggcggctgaggagcacgta | gcggcacaagcagcgttgg |
mouse Atrogin1 | cagcttcgtgagcgacctc | ggcagtcgagaagtccagtc |
mouse Murf1 | gtgtgaggtgcctacttgctc | gctcagtcttctgtccttgga |
mouse Lc3b | aagcagcgccggagctttga | gagctgcaagcgccgtctga |
mouse Atg5 | gtggtttggacgaattccaac | agagctgaacttgatgcaaga |
mouse Atg7 | cacggttcgataatgttcttcc | gaatccttctcgctcgtactg |
mouse Atg16l | ccaggaggcgtcaagcacgg | tgctgacagctcggacggga |
mouse Bmp2 | gtcgaagctctcccactgac | caggaagctttgggaaacag |
mouse Col1a1 | cttgccagcttccccatcatct | catgggtccttctggtcctcgt |
mouse Osx | aacttcttctcccgggtgtg | tgaggaagaagcccattcac |
mouse Sost | gtgtgatgttgggctacgtg | ccaccacaatctctccccta |
mouse Rank | tgctcctcttcatctctgtggtagtagtgg | tggagtgaagtctgcgccccaccctgctcc |
mouse Rankl | aggctgggccaagatctcta | gtctgtaggtacgcttcccg |
mouse Opg | agcaggagtgcaaccgcacc | ttccagcttgcaccacgccg |
mouse Actb | ccccattgaacatggcattg | acgaccagaggcatacagg |
mouse 18 s | tcaagaacgaaagtcggagg | ggacatctaagggcatcac |
mouse Hprt | tcagtcaacgggggacataa | ggggctgtactgcttaaccag |
