Figure 1
From: Propidium iodide staining underestimates viability of adherent bacterial cells
Epifluorescence microscopy images of adherent E. coli (a,c,e) and S. epidermidis (b,d,f) viability staining. 24 h initial monolayer biofilm formed on glass in PBS stained in situ with propidium iodide (PI) and SYTO 9 (a,b), with fluorescein diacetate (FDA) (c,d) or harvested via sonication, stained with PI and SYTO 9 and collected on filter (e,f). Pie diagrams represent total cell count on surfaces with PI, SYTO 9 and FDA stained signal proportions marked in red, dark green and light green respectively. Scale bars correspond to 10 µm.
