Figure 3
Effect of EGF on TGF-βRII in primary human corneal epithelial cells (pHCE). Protein (A) and mRNA (B) expression of TGF-βRII was examined in pHCE treated ± EGF for 3 or 6 hours (hr). Data was reported as relative fold with samples normalized to Control (C: no treatment), which was set to 1, and representative western blot (A) and RT-PCR (B) were included. G3PDH served as an internal control for RT-PCR. For both protein and mRNA, 6 hours of EGF treatment significantly increased the amount of TGF-βRII in pHCE (protein: 1.8 fold; mRNA: 8.3 fold; *p < 0.05). Original western blot and PCR gel presented are available in Supplemental Fig. S2.
