Figure 5
EGF and TGFβ1 time course for p38 and ERK1/2 phosphorylation. Representative western blots of pHCE incubated ± TGFβ1 or EGF for 24 hours and examined for (A) p-p38 and p38 or (B) pERK1/2 and ERK1/2. The ratios (p-p38/p38 or pERK1/2/ERK1/2) were normalized to 0 m (no treatment), which was set to 1, and reported as relative fold. (A) TGFβ1 stimulated the upregulation of p-p38 in a cyclical manner and was significantly (*p < 0.05) increased as compared to 0 m at 24 hrs, whereas EGF had no apparent effect on p-p38 levels. (B) EGF significantly stimulated the upregulation of pERK1/2 by 5 m (*p < 0.05) and 10 m (**p < 0.01), peaking at 15 m (**p < 0.05), while TGFβ1 did not activate ERK1/2. Original western blots presented are available in Supplemental Fig. S4.
