Figure 4
Classification of PaPYL4–8 subfamilies. A yeast two-hybrid interaction assay was performed between putative P. aegyptiaca ABA receptors fused to a binding domain and A. thaliana ABA co-receptor ABI1 and its mutant ABI1G180D fused to an activating domain, in the presence of Mock (0.1% DMSO) (left) or 10 µM ABA (right). The models of the defining differences between the ABA receptor subfamilies are represented by PYR1 (subfamily III), PYL4 (subfamily II) and PYL9 (subfamily I), each fused to a binding domain. See methods for phylogenetic analyses experimental procedures.
