Figure 1
Rik-203 regulates neural differentiation. (A) Rik-203 levels in different tissues of mice, detected by RT-PCR. The hippocampus has the highest level of Rik-203. (B) Microarray studies revealed the increase of Rik-203 expression during the neural differentiation from ESCs to NPCs. (C) The increase of Rik-203 mRNA levels during the neural differentiation from ESCs to NPCs was confirmed by RT-PCR at day 3, 5 and 7 after the induction of neural differentiation. (D) knockdown of Rik-203 decreased levels of Rik-203. (E) Measurement of Sox1 positive cells indicated that knockdown of Rik-203 decreased the number of Sox1 positive cells. (F) The quantification of Sox1 positive cells using FACS showed that knockdown of Rik-203 decreased the number of Sox1 positive cells. (G) RT-PCR showed that the mRNA levels of Sox1 and Nestin were decreased through knockdown of Rik-203. The scale bar represents 100 um. FACS: Fluorescent-activated cell sorting. Rik-203: C130071C03Rik; ESCs: Embryonic Stem Cells; NPCs: Neural Precursor Cells; shRNA: Short hairpin RNA; GFP: Green Fluorescent Protein; Dox: Doxcycline. Ctrl: control; Sox1: SRY (sex determining region Y)-box 1. The data were presented as mean + standard deviation (SD) with three independent experiments. *p < 0.05, **p < 0.01, ***P < 0.001; by one-way ANOVA (A,C,D,F,G).
