Table 1 Peptide sequences of the validated NES motifs or false positives used in the structure-based modeling.

From: Structural prerequisites for CRM1-dependent nuclear export signaling peptides: accessibility, adapting conformation, and the stability at the binding site

Protein	Class	NES sequence	K_D (nM)^§	ref.
MVM NS2	1a	₇₇STVDEMTKKFGTLTIHD₉₃	2	³¹
^*super PKI	1a	₃₄NLNELALKLAGLDINK₄₉	4	³¹
PKI	1a	₃₄NSNELALKLAGLDINK₄₉	34	³¹
ADAR1	1a	₁₂₁RGVDCLSSHFQELSIYQ₁₃₇	69	³¹
MEK1	1a	₂₈TNLEALQKKLEELELDE₄₄	70	³¹
Pax	1a	₂₆₄RELDELMASLSDFKFMA₂₈₀	700	³¹
^*CPEB4-R	1a	₃₉₅RMIDILSSELSHMDFTR₃₇₉	710	³¹
NPMmutA	1a	₂₇₈MTDQEAIQDLCLAVEEVSLRK₂₉₈	790	³¹
HDAC5	1a	₁₀₈₁EAETVSAMALLSVG₁₀₉₅	1600	³¹
p73	1a	₃₆₄NFEILMKLKESLELMELVP₃₈₂	2000	³¹
^*hRio2-R	1a	₄₀₅GKIEELAQNFETMEFSR₃₈₉	2600	³¹
Stradα	1a	₄₁₃GIFGLVTNLEELEVD₄₂₇	10300	³¹
^*FMRP-1b	1b	YLKEVDQLRALERLQID	3000	¹⁴
SNUPN	1c	₁MEELSQALASSFSVSQDLNS₂₀	12500	³¹
HPV E7	1c	₇₃HVDIRTLEDLLMGTLGIVC₉₁	34000	³¹
HIV Rev	2	₇₃LQLPPLERLTLDC₈₅	1180	³¹
FMRP	2	₄₂₄LKEVDQLRLERLQID₄₃₈	2000	³¹
SMAD4	2	₁₃₄ERVVSPGIDLSGLTLQ₁₄₉	4600	³¹
mDia2	3	₁₁₅₇SVPEVEALLARLRAL₁₁₇₁	1600	³¹
CDC7	3	₄₅₆QDLRKLCERLRGMDSSTP₄₇₃	20000	³¹
X11L2	4	₅₅SSLQELVQQFEALPGDLV₇₂	1500	³¹
CPEB4	1a-R	₃₇₉RTFDMHSLESSLIDIMR₃₉₅	800	³¹
hRio2	1a-R	₃₈₉RSFEMTEFNQALEEIKG₄₀₅	2800	³¹
^*PKImut1 (I47A)	—	₃₄NSNELALKLAGLDANK₄₉	150000	³¹
^*PKImut2 (L42A/L45A)	—	₃₄NSNELALKAAGADINK₄₉	900000	³¹
^†APC	1a	₁₆₃AQLQNLTKRIDSLPL₁₇₄	(−)	³³
^‡Cyclin D1	1a	₂₈₁VDLACTPTDVRDVDI₂₉₅	(−)	—
APRIL	1b	₁₀₆LEPLKKLECLKSLDL₁₂₀	(−)	³³
^‡hTERT	1c	₉₆₅KAGRNMRRKLFGVLRLKC₉₈₂	(−)	—
DcpS	1c	₁₃₆TEKHLQKYLRQDLRL₁₅₀	(−)	³³
Cdk5	2	₁₃₃LINRNGELKLADFGL₁₄₇	(−)	³³
^†FGF1	2	₁₃₈THYGQKAILFLPLPV₁₅₂	(−)	³³
COMMD1	3	₁₇₁ILKTLSEVEESISTL₁₈₅	(−)	²⁰
DEAF1	1a-R	₄₅₂SWLYLEEMVNSLLNTAQQ₄₆₉	(−)	¹³
SGN5	1a-R	₂₂₁YALEVSYFKSSLDRKLL₂₃₈	(−)	¹³
^†COMMD1–2	1a-R	₁₆₄DEVKVNQILKTLSEVEES₁₈₁	(−)	¹³
^†ELF3	1a-R	₁₁₁RLVFGPLGDQLHAQLR₁₂₆	(−)	¹³

^*Engineered or mutated (underscored residues are the ones inserted or mutated).
^†Do not fit the consensus in Fig. 1 (due to Pro or do not have a bulky residue at Φ3/4 in the class 1a-R).
^‡Unpublished data.
^§(−) means no binding determined by pull-down binding assay.

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Table 1 Peptide sequences of the validated NES motifs or false positives used in the structure-based modeling.

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