Figure 5
Oncolytic MV and CPT combinatorial treatment causes cell cycle arrest and induction of apoptosis in human MCF-7 breast cancer cells. (A) Flow cytometric cell cycle assay was performed using propidium iodide (PI) staining on MCF-7 cells following 5 days of treatment with CPT and MV in co-treatment model. Percentage of cells in sub G1, G0/G1, S or G2 phase were calculated by Beckman Cytomics FC500 Flow Cytometry CXP analysis. (B) Flow cytometric double stain assay was performed using propidium iodide (PI) and Annexin V (APC conjugated) staining on MCF-7 cells following 5 days of treatment with CPT and MV in co-treatment model. Percentage of cells that were necrotic, early apoptotic, late necrotic, or live was determined by Beckman Cytomics FC500 Flow Cytometry CXP analysis. Data shown are means ± SEM from three independent experiments.
