Figure 2
Control of data quality. (a) Venn-diagram represents intersection of proteins identified from total lysate and enriched-mitochondrial fraction of the 28 liver samples, with 645 proteins localized in mitochondria according to MitoCarta2.0 mouse inventory. (b) Heat-map shows the stronger relative intensity of 65 OXPHOS enzymes in the mitochondria-enriched fractions compared with cellular lysate. (c) After very short membrane exposure (t = 5 s) by chemiluminescence, Western Blot of five OXPHOS enzymes visible only in concentrated mitochondrial fraction (lanes 1 and 2) and not in cellular lysate (lanes 3 and 4). Full membrane picture available in Supplementary Fig. 7. A longer exposure in Supplementary Fig. 2a represents gradual appearance of OXPHOS components in cellular lysate. (d) The average mtFE score for protein markers of subcellular compartments: mitochondrion, peroxisome, ER, cytosol, nucleus, and spliceosome (e–j). The violin plots in dark violet present separately the difference in the distributions of mtFE scores of selected protein markers: (e) mitochondrion, (f) peroxisome, (g) ER, (h) cytosol, (i) nucleus, and (j) spliceosome. The violin plots in white color present distributions of all other measured proteins. n corresponds to the number of the respective subcellular markers per individual violin plot in dark violet.
