Figure 2
Structure and decoding result of the DNA-based data storage platform. We achieved the highest information capacity and physical density of DNA-based data storage. (A) Design structure of DNA fragments. (B) DNA fragments can be analyzed using NGS. After classification by address, degenerate bases can be decoded by examining the distribution of characters in the same position (yellow bar). (C) Degenerate bases can be determined from the scatter plot of the ratio of bases in the same position. (D) The error rate of determined DNA bases in specific average coverage of the total fragments. The standard deviations (s.d.) were obtained by repeating the random sampling 10 times. The error bars represent s.d. (E) Summary of the experimental results. The information capacity is calculated from the input information in bits divided by the number of encoding characters (excluding that of adapter sites). We compared the results of our work with those of Erlich and Zielinski10, who previously reported the highest information capacity and physical density using pooled oligo synthesis and high-throughput sequencing data. The physical density is the ratio of the number of bytes encoded to the weight of the DNA library used to decode the information.
