Figure 2

SDS-PAGE analysis of TreS from different recombinant strains. Lane M, protein molecular mass marker; Lane 1, intracellular proteins of recombinant B. subtilis W800N-Pgrac; Lane 2, intracellular proteins of recombinant B. subtilis W800N-Pglv; Lane 3, intracellular proteins of recombinant B. subtilis W800N (ΔamyE)-Pglv; Lane 4. proteins in supernatant of recombinant B. subtilis W800N-Pglv fermentation broth; and Lane 5, proteins in supernatant of recombinant B. subtilis W800N (ΔamyE)-Pglv fermentation broth. TreS is indicated by an arrow. Molecular mass markers indicated from top to bottom.