Table 1 Strains, plasmids, and primers used in this study.
Relevant properties and Sequence (5′→3ʹ) | Source | |
|---|---|---|
Strains | ||
E. coli DH5α | deoR endA1 gyrA96 hsdR17 supE44 thi1 recA1 lacZM15 | Laboratory collection |
B. subtilis 168 | trpC2 | Laboratory collection |
B. subtilis W800N | nprE aprE epr bpr mpr:: ble nprB:: bsr ∆vpr wprA:: hyg cm:: neo; NeoR | Laboratory collection |
Plasmids | ||
pHT01 | Plasmid-based expression vector for B. subtilis containing IPTG-inducible Pgrac promoter, CmR | Laboratory collection |
pHT01-Pglv-treS | Pglv promoter instead of Pgrac promoter | This study |
pHT01-Pglv-treS ʹ | Cre sequence (AT base instead of CG base) in Pglv promoter | This study |
Primers | ||
Pglv-1-F | CGCGGATCCGGCATGTATCCGAATCGT the underlined site is BamHI | This study |
Pglv-1-R-C | CATATGACGACCTCCTTGATAAATTTTACAATTCCATTTATACCATGA | This study |
Pglv-2-F-C | TGTAAAATTTATCAAGGAGGTCGTCATATGAA | This study |
Pglv-2-R-C | TAATAGCGGGCGGAGGGAGCACTTTCACTCCATA AATGAAATTCCCCCATTTTCGAATCTGTAATTGT | This study |
TreS-F-C | TTCCCCCATTTTCGAATCTGTAATTGTATATAATAGAAAGAAAATGGGGGGATCTGATATGACCCAGCCCGACCCGT | This study |
TreS-R | CCCAAAGACGTCTCAAACATGCCCGCTGC the underlined site is AatII | This study |
Spe-F | TGGAAACACACAGTGAGGAGGATATATTTGAATACACACGAACA | This study |
Spe-R | CGCGGATCCTTATAATTTTTTTAATCCGTTATTTAAATAGTT | This study |
amyE-1-F | CGCGGATCCATGTTTGCAAAACGATTCAAAACCT the underlined site is BamHI | This study |
amyE-1-R | CAAATATATCCTCCTCACTGTGTGTTTCCATGTGTCCAGTTT | This study |
