Figure 5

Representative western blot of total eNOS (top blot), eNOS phosphorylation at Serine1177 (top blot), caveolin-1(top blot), and GAPDH (bottom blot; loading control) in normotensive rat and SHR aortas (A,C,E) and mesenteric arteries (B,D,F) treated with methyl-β-cyclodextrin (10 mM dextrin, for 60 min) or not (Control). We processed images of blots changing brightness and contrast and we applied equally over the entire image and controls. *p < 0.05, **p < 0.01, ***p < 0.001 statistical difference between Dextrin versus Control groups; #p 0.05 statistical difference between SHR versus normotensive rat groups.