Figure 1
From: Reduced Basal Nitric Oxide Production Induces Precancerous Mammary Lesions via ERBB2 and TGFβ
Non-malignant mammary epithelial cells produce the basal level of nitric oxide (NO) in 3D ECM cultures, whereas the level plummets along with breast cancer progression. (A) (Left) Cell lines of MCF10A breast cancer progression series (10A; non-malignant; AT1: premalignant; DCIS: ductal carcinoma in situ; and CA1d: malignant)74,75,76,77,78,79,80 cultured with 5% Matrigel drip and stained with the fluorescent NO probe DAF-FM DA. (Right) Quantification of DAF-FM DA signal/cell in the series. (See DAF-FM DA signals of a panel of normal vs. cancerous MECs in Supplementary Fig. 1A,B). (B) The level of NO metabolite nitrite in the conditioned media of the progression series. (C) (Left) Cell lines of the progression series stained with an antibody against S-nitrosocysteine (S-NOC). (Right) Quantification of S-NOC level in the nucleus (N) vs. cytosol (C) of the series. AU: arbitrary unit. Error bars: mean ± STDEV. *p < 0.05; **p < 0.01; and ***p < 0.001.
