Figure 1

Effects of ERRα in cancer cells on ERRα and TGF-β in stromal cells. (A) The expression of ERRα and PGC-1α in Ishikawa and HEC-1A cells was examined by qPCR. (B) T-HESCs were co-cultured with Ishikawa or HEC-1A cells that had been transfected with ERRα/PGC-1α expression plasmids or silenced for ERRα expression. The expression of ERRα (C) and TGF-β (D) was measured using qPCR, in T-HESCs co-cultured with cancer cells overexpressing ERRα/PGC-1α. (E) The effect of ERRα knockdown was evaluated by qPCR and western blot analysis. (F) TGF-β expression was examined in T-HESCs co-cultured with cancer cells with ERRα knockdown. Data represent the mean ± standard error of the mean. P values are based on Student’s t-test. **P < 0.01.