Figure 3

Effects of ERRα on TGF-β-induced EMT markers. The expression of ERRα, vimentin, Snail, and ZEB1 was examined in Ishikawa (A) and HEC-1A (B) cells mono-cultured in the presence of 10 ng/mL TGF-β, after overexpression of ERRα/PGC-1α. Western blot analysis of E-cadherin, vimentin, Snail, and ZEB1 in cancer cells treated with TGF-β, after overexpression of ERRα/PGC-1α (C,D). Western blot analysis of these EMT-associated factors in both cancer cell types with ERRα knockdown (E and F). Full-length blots are presented in Supplementary Figs 1–4. Data represent the mean ± standard error of the mean. P values are based on Student’s t-test. **P < 0.01.